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Differential expression of keratin gene family members in the TCGA-LUAD cohort. A, Heatmap of 17 keratin family genes in normal and tumor using the TCGA-LUAD database. B–F, High mRNA expression levels of five prognostic keratin genes ( <t>KRT7</t> , KRT8 , KRT17 , KRT18 and KRT80 ) in LUAD vs. normal tissues (TCGA database).
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Differential expression of keratin gene family members in the TCGA-LUAD cohort. A, Heatmap of 17 keratin family genes in normal and tumor using the TCGA-LUAD database. B–F, High mRNA expression levels of five prognostic keratin genes ( <t>KRT7</t> , KRT8 , KRT17 , KRT18 and KRT80 ) in LUAD vs. normal tissues (TCGA database).
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Image Search Results


Differential expression of keratin gene family members in the TCGA-LUAD cohort. A, Heatmap of 17 keratin family genes in normal and tumor using the TCGA-LUAD database. B–F, High mRNA expression levels of five prognostic keratin genes ( KRT7 , KRT8 , KRT17 , KRT18 and KRT80 ) in LUAD vs. normal tissues (TCGA database).

Journal: Heliyon

Article Title: Keratin gene signature expression drives epithelial-mesenchymal transition through enhanced TGF-β signaling pathway activation and correlates with adverse prognosis in lung adenocarcinoma

doi: 10.1016/j.heliyon.2024.e24549

Figure Lengend Snippet: Differential expression of keratin gene family members in the TCGA-LUAD cohort. A, Heatmap of 17 keratin family genes in normal and tumor using the TCGA-LUAD database. B–F, High mRNA expression levels of five prognostic keratin genes ( KRT7 , KRT8 , KRT17 , KRT18 and KRT80 ) in LUAD vs. normal tissues (TCGA database).

Article Snippet: The small interfering RNAs (siRNA) designed to target KRT7 (si- KRT7 ) and KRT8 (si- KRT8 ), along with non-specific control siRNA (si-Control) were synthesized by RiboBio (Guangzhou, China).

Techniques: Quantitative Proteomics, Expressing

Prognostic value of mRNA expression of KRT family members in the TCGA-LUAD cohort. Survival curves comparing the high and low expression of KRT7 (A), KRT8 (B), KRT17 (C), KRT18 (D) and KRT80 (E) in TCGA-LUAD.

Journal: Heliyon

Article Title: Keratin gene signature expression drives epithelial-mesenchymal transition through enhanced TGF-β signaling pathway activation and correlates with adverse prognosis in lung adenocarcinoma

doi: 10.1016/j.heliyon.2024.e24549

Figure Lengend Snippet: Prognostic value of mRNA expression of KRT family members in the TCGA-LUAD cohort. Survival curves comparing the high and low expression of KRT7 (A), KRT8 (B), KRT17 (C), KRT18 (D) and KRT80 (E) in TCGA-LUAD.

Article Snippet: The small interfering RNAs (siRNA) designed to target KRT7 (si- KRT7 ) and KRT8 (si- KRT8 ), along with non-specific control siRNA (si-Control) were synthesized by RiboBio (Guangzhou, China).

Techniques: Expressing

In vitro studies on KGS signature genes in LUAD tumorigenesis. A, The mRNA expression level of KRT7 and KRT8 in human lung cells (NCI–H1299 and A549 cell lines). B, The mRNA expression level of KRT7 and KRT8 in NCI–H1299 and A549 cells transfected with si- KRT7 , si- KRT8 , and si-Control. C, Proliferation curves assessed by CCK8 assay during 120 h for KRT7 knockdown cell model of NCI–H1299/A549 cells. D, Proliferation curves assessed by CCK8 assay during 120 h for KRT8 knockdown cell model of NCI–H1299 and A549 cell lines. E, The cloning ability for KRT7 and KRT8 knockdown cell models of NCI–H1299 and A549 cell lines. F, The expression level of TGF-β pathway-related genes in NCI–H1299 and A549 cell lines with si- KRT7 . G, The expression level of TGF-β pathway-related genes in NCI–H1299 and A549 cell lines with si- KRT8 .

Journal: Heliyon

Article Title: Keratin gene signature expression drives epithelial-mesenchymal transition through enhanced TGF-β signaling pathway activation and correlates with adverse prognosis in lung adenocarcinoma

doi: 10.1016/j.heliyon.2024.e24549

Figure Lengend Snippet: In vitro studies on KGS signature genes in LUAD tumorigenesis. A, The mRNA expression level of KRT7 and KRT8 in human lung cells (NCI–H1299 and A549 cell lines). B, The mRNA expression level of KRT7 and KRT8 in NCI–H1299 and A549 cells transfected with si- KRT7 , si- KRT8 , and si-Control. C, Proliferation curves assessed by CCK8 assay during 120 h for KRT7 knockdown cell model of NCI–H1299/A549 cells. D, Proliferation curves assessed by CCK8 assay during 120 h for KRT8 knockdown cell model of NCI–H1299 and A549 cell lines. E, The cloning ability for KRT7 and KRT8 knockdown cell models of NCI–H1299 and A549 cell lines. F, The expression level of TGF-β pathway-related genes in NCI–H1299 and A549 cell lines with si- KRT7 . G, The expression level of TGF-β pathway-related genes in NCI–H1299 and A549 cell lines with si- KRT8 .

Article Snippet: The small interfering RNAs (siRNA) designed to target KRT7 (si- KRT7 ) and KRT8 (si- KRT8 ), along with non-specific control siRNA (si-Control) were synthesized by RiboBio (Guangzhou, China).

Techniques: In Vitro, Expressing, Transfection, Control, CCK-8 Assay, Knockdown, Cloning